Ge J, Bratlie A, Hannestad K
Department of Immunology, University of Tromsø, School of Medicine, Norway.
Tissue Antigens. 1994 Nov;44(5):306-10. doi: 10.1111/j.1399-0039.1994.tb02400.x.
TrJ14 is a cytotoxic human IgG1 lambda hybridoma mAb that recognized a novel HLA-A epitope expressed by lymphoblastoid B cells that are homo- or heterozygous for A2, A3, A11, A30, A31, A33, A68 and A69. Based on these results, the HLA type of cell line TEM (10w9057) was retyped as A66. When peripheral blood T cells isolated freshly from 265 HLA-typed normal individuals served as targets, TrJ14 killed cells expressing two TrJ14-positive HLA-A alleles, as well as the majority of cells having one TrJ14-positive and one TrJ14-negative HLA-A antigen. However, TrJ14 failed to recognize or reacted weakly with most HLA-A2 and -A3 heterozygous normal T cells when A2 or A3 was coexpressed together with a TrJ14-negative antigen. The serological reactivity of TrJ14 correlated with the amino acid valine and aspartic acid at positions 76 and 77 of the alpha 1-domain helix. These amino acids were shared exclusively by all the identified TrJ14+ alleles.
TrJ14是一种具有细胞毒性的人IgG1λ杂交瘤单克隆抗体，它识别一种由淋巴母细胞样B细胞表达的新型HLA - A表位，这些B细胞对于A2、A3、A11、A30、A31、A33、A68和A69为纯合子或杂合子。基于这些结果，细胞系TEM（10w9057）的HLA类型被重新分型为A66。当从265名经HLA分型的正常个体中新鲜分离的外周血T细胞作为靶细胞时，TrJ14可杀伤表达两个TrJ14阳性HLA - A等位基因的细胞，以及大多数具有一个TrJ14阳性和一个TrJ14阴性HLA - A抗原的细胞。然而，当A2或A3与TrJ14阴性抗原共表达时，TrJ14无法识别大多数HLA - A2和 - A3杂合正常T细胞或与之发生微弱反应。TrJ14的血清学反应性与α1结构域螺旋第76和77位的氨基酸缬氨酸和天冬氨酸相关。所有已鉴定的TrJ14 +等位基因都仅共享这些氨基酸。
