一种针对HLA - B44(12)和 - B45(12)的细胞毒性单克隆人杂交瘤抗体（TrJ3）。

A cytotoxic monoclonal human hybridoma antibody (TrJ3) against HLA-B44(12) and -B45(12).

作者信息

Ge J, Bratlie A, Hannestad K

机构信息

Department of Immunology, University of Tromsø, School of Medicine, Norway.

出版信息

Tissue Antigens. 1992 May;39(5):258-61. doi: 10.1111/j.1399-0039.1992.tb01944.x.

DOI:10.1111/j.1399-0039.1992.tb01944.x

PMID:1384167

Abstract

We have generated a human monoclonal cytotoxic IgM lambda antibody (TrJ3) that reacted specifically with all lymphoblastoid B-cell lines expressing HLA-B44(12) and B45(12). TrJ3 hybridoma supernatant was suitable for HLA-B12 typing of freshly isolated blood mononuclear cells. Analysis of available amino acid sequences of HLA-B molecules indicated that the alpha 1 domain does not contain the TrJ3 serological epitope. Since HLA-B44 is associated with a unique serine residue at position 167 that points towards the peptide binding groove, we propose that S167 of the alpha 2 domain helix is a critical part of the TrJ3 epitope.

摘要

我们已经产生了一种人源单克隆细胞毒性IgMλ抗体（TrJ3），它能与所有表达HLA - B44(12)和B45(12)的淋巴母细胞B细胞系发生特异性反应。TrJ3杂交瘤细胞上清液适用于对新鲜分离的血液单核细胞进行HLA - B12分型。对HLA - B分子可用氨基酸序列的分析表明，α1结构域不包含TrJ3血清学表位。由于HLA - B44与位于第167位的一个独特丝氨酸残基相关，该丝氨酸残基指向肽结合槽，我们提出α2结构域螺旋的S167是TrJ3表位的关键部分。