一种对HLA - B38(16)和 - B39(16)具有特异性的细胞毒性人杂交瘤单克隆抗体（TrJ5）。

A cytotoxic human hybridoma monoclonal antibody (TrJ5) specific for HLA-B38(16) and -B39(16).

作者信息

Ge J, Hannestad K

机构信息

Department of Immunology, University of Tromsø School of Medicine, Norway.

出版信息

Hum Immunol. 1993 Mar;36(3):168-71. doi: 10.1016/0198-8859(93)90121-g.

DOI:10.1016/0198-8859(93)90121-g

PMID:7686545

Abstract

We have generated a cytotoxic IgM lambda human hybridoma mAb (TrJ5), that is specific for HLA-B38(16) and -B39(16). Among a panel of 42 HLA-defined cell lines, TrJ5 killed all five B38-positive and both B16-positive cell lines, as well as the single B39-positive cell line, but not any cell lines lacking these antigens. It could be ruled out that the TrJ5 epitope is located on the alpha 1 domain because TrJ5 did not react with cells bearing HLA-B14, the alpha 1 domain of which is identical to alpha 1 of B39. In the alpha 2 domain of B38 and B39, a unique threonine residue at position 158 (Thr-158) is not shared by any of 29 other HLA-B alleles. This suggests that Thr-158 of the alpha 2-domain helix is critical for the TrJ5 epitope.

摘要

我们已制备出一种细胞毒性IgM λ型人杂交瘤单克隆抗体（TrJ5），它对HLA - B38(16)和 - B39(16)具有特异性。在一组42个HLA分型的细胞系中，TrJ5杀死了所有5个B38阳性细胞系、2个B16阳性细胞系以及唯一的B39阳性细胞系，但对任何缺乏这些抗原的细胞系均无作用。可以排除TrJ5表位位于α1结构域，因为TrJ5不与携带HLA - B14的细胞反应，HLA - B14的α1结构域与B39的α1结构域相同。在B38和B39的α2结构域中，位置158处的独特苏氨酸残基（Thr - 158）在其他29个HLA - B等位基因中均不存在。这表明α2结构域螺旋的Thr - 158对于TrJ5表位至关重要。