确定肠炎沙门氏菌鞭毛蛋白g,m血清型的结构域。
Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis.
作者信息
van Asten A J, Zwaagstra K A, Baay M F, Kusters J G, Huis in't Veld J H, van der Zeijst B A
机构信息
Department of Bacteriology, University of Utrecht, The Netherlands.
出版信息
J Bacteriol. 1995 Mar;177(6):1610-3. doi: 10.1128/jb.177.6.1610-1613.1995.
Abstract
Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis.
摘要
构建了表达肠炎沙门氏菌鞭毛蛋白片段的克隆，并使用一种g,m特异性单克隆抗体进行筛选。结果表明，g,m表位位于鞭毛蛋白的第258至348个氨基酸之间。编码肠炎沙门氏菌鞭毛蛋白的fliC基因被证明是肠炎沙门氏菌中唯一存在的鞭毛蛋白基因。
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