Itoh H, Ide H, Kataoka H, Tomita M, Yoshihara H, Nawa Y
Department of Parasitology, Miyazaki Medical College.
J Biochem. 1994 Oct;116(4):767-72. doi: 10.1093/oxfordjournals.jbchem.a124594.
A cDNA encoding alpha 1-microglobulin (alpha 1mG)/inter-alpha-trypsin inhibitor light chain (ITI-LC) was cloned from mouse liver by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. Sequence analysis of the cDNA showed that the basic molecular structure of the proprotein was similar to that in other animals, so that two mature proteins, alpha 1mG and ITI-LC, could be produced from the proprotein translated from the mRNA. Since ITI-LC is known as a positive acute phase reactant and since ITI-LC is genetically identical with mast cell proteinase inhibitor, trypstatin, we examined the mRNA level in the liver of parasite-infected mice showing extensive mastocytosis. The mRNA level was, however, not significantly changed during inflammatory processes, except for a slight increase on day 8 post-infection.
通过逆转录-聚合酶链反应和cDNA末端快速扩增，从小鼠肝脏中克隆出编码α1-微球蛋白（α1mG）/间α-胰蛋白酶抑制剂轻链（ITI-LC）的cDNA。对该cDNA的序列分析表明，前体蛋白的基本分子结构与其他动物相似，因此从mRNA翻译的前体蛋白可产生两种成熟蛋白，即α1mG和ITI-LC。由于ITI-LC是一种阳性急性期反应物，且ITI-LC与肥大细胞蛋白酶抑制剂胰蛋白酶抑制素在基因上相同，我们检测了寄生虫感染小鼠肝脏中显示广泛肥大细胞增多的mRNA水平。然而，除感染后第8天略有增加外，炎症过程中mRNA水平没有显著变化。
