Tomita M, Itoh H, Ishikawa N, Higa A, Ide H, Murakumo Y, Maruyama H, Koga Y, Nawa Y
Department of Parasitology, Miyazaki Medical College, Japan.
Biochem J. 1995 Oct 1;311 ( Pt 1)(Pt 1):293-7. doi: 10.1042/bj3110293.
A cDNA encoding mouse intestinal trefoil factor (mITF) was successfully cloned and sequenced from the small intestine of C57BL/6 mouse by using the combination of reverse transcription-PCR and rapid amplification of cDNA ends methods. The gene was, similar to rat and human ITFs, mainly expressed in the small and large intestine. The mITF expression was up-regulated during the recovery phase after depletion of goblet cells in acetic acid-induced colitis. On the other hand, the expression in the jejunum was not altered, while goblet cell hyperplasia was induced by Nippostrongylus brasiliensis infection. These results suggest that the mITF expression did not simply correlate with the number of goblet cells. The mITF may play an important role in the maintenance and repair of mucosal function of the rectum. Additionally, the mITF in the jejunum may play a role in alteration of the physicochemical nature of goblet cell mucins, thereby affecting the establishment of intestinal helminths.
通过逆转录聚合酶链反应（RT-PCR）和cDNA末端快速扩增（RACE）方法相结合，成功地从C57BL/6小鼠的小肠中克隆并测序了编码小鼠肠三叶因子（mITF）的cDNA。与大鼠和人类ITF相似，该基因主要在小肠和大肠中表达。在乙酸诱导的结肠炎中杯状细胞耗竭后的恢复阶段，mITF表达上调。另一方面，在巴西日圆线虫感染诱导杯状细胞增生时，空肠中的表达未发生改变。这些结果表明，mITF的表达并不简单地与杯状细胞的数量相关。mITF可能在直肠黏膜功能的维持和修复中起重要作用。此外，空肠中的mITF可能在杯状细胞黏蛋白的物理化学性质改变中起作用，从而影响肠道蠕虫的定植。
