Shliapnikov M G, Ksenzenko V N
Mol Biol (Mosk). 1994 Nov-Dec;28(6):1321-9.
Nucleotide sequence of 24 genes for the bacteriophage T5 tRNAs specific for all amino acids involved in protein synthesis was determined. All of them, except tRNA(Pro), were shown to differ significantly from the generalized "clover-leaf" structure consisting in the displacement of invariant and semi-invariant residues, the absence of pairing in the stems and some other deviations from the canonical parameters of the model. Basing on the available information on the functional activity of the phage-specific tRNAs, one can put forward a suggestion that, at least for some of them, the above anomalies do not essentially influence their activity. A comparison of phage T5 tRNAs with the Escherichia coli and phage T4 counterparts was carried out. The majority of the known elements determining the specificity of E. coli tRNA aminoacylation was also found in all phage T5 tRNAs, except tRNAPhe).
测定了噬菌体T5中与蛋白质合成所涉及的所有氨基酸对应的24种tRNA的核苷酸序列。除了tRNA(Pro)之外，所有这些tRNA均显示出与广义的“三叶草”结构存在显著差异，包括不变和半不变残基的位移、茎中配对的缺失以及其他一些偏离模型标准参数的情况。基于关于噬菌体特异性tRNA功能活性的现有信息，可以提出这样一种建议，即至少对于其中一些tRNA而言，上述异常情况并未对其活性产生实质性影响。对噬菌体T5的tRNA与大肠杆菌和噬菌体T4的相应tRNA进行了比较。除了tRNAPhe)之外，在所有噬菌体T5的tRNA中也发现了大多数决定大肠杆菌tRNA氨基酰化特异性的已知元件。
