Engbaek K, Koch C
Department of Immunology, Statens Seruminstitut, Copenhagen, Denmark.
APMIS. 1994 Dec;102(12):931-42.
The soluble antigens of Rochalimaea henselae, Rochalimaea quintana and Afipia felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. A precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. By this technique 56 antigens were identified from R. henselae, 49 from R. quintana, and 39 from A. felis. The serological cross-reaction between R. henselae, R. quintana and A. felis, and between these 3 bacteria and 32 pathogenic bacteria was analysed by rocket-line immunoelectrophoresis, crossed-line immunoelectrophoresis, and tandem-crossed electrophoresis. It was concluded that (i) 4-7 antigens distinguish R. henselae, R. quintana and A. felis from each other, (ii) both Gram-positive and Gram-negative bacteria cross-react with R. henselae, R. quintana and A. felis antisera, (iii) the cross-reacting antigens of Gram-negative bacteria have both precipitating and non-precipitating specificities, whereas Gram-positive bacteria have mainly non-precipitating specificities, (iv) the cross-reacting antigens are common to several species, and (v) fewer cross-reacting antigens are found in phylogenetically disparate species than in more closely related species.
以细菌超声裂解物作为抗原，与混合的超免疫兔血清进行交叉免疫电泳，以此来鉴定汉氏罗卡利马氏体、五日罗卡利马氏体和猫阿菲披亚菌的可溶性抗原。为每种细菌绘制了沉淀素图谱，结果表明即使在中间凝胶中加入正常或免疫前兔血清，该图谱也是可重复且稳定的。通过这项技术，从汉氏罗卡利马氏体中鉴定出56种抗原，从五日罗卡利马氏体中鉴定出49种抗原，从猫阿菲披亚菌中鉴定出39种抗原。通过火箭免疫电泳、交叉免疫电泳和串联交叉电泳分析了汉氏罗卡利马氏体、五日罗卡利马氏体和猫阿菲披亚菌之间，以及这三种细菌与32种病原菌之间的血清学交叉反应。得出的结论如下：（i）4 - 7种抗原可区分汉氏罗卡利马氏体、五日罗卡利马氏体和猫阿菲披亚菌；（ii）革兰氏阳性菌和革兰氏阴性菌均与汉氏罗卡利马氏体、五日罗卡利马氏体和猫阿菲披亚菌抗血清发生交叉反应；（iii）革兰氏阴性菌的交叉反应抗原具有沉淀和非沉淀特异性，而革兰氏阳性菌主要具有非沉淀特异性；（iv）交叉反应抗原在多个物种中是共有的；（v）在系统发育关系较远的物种中发现的交叉反应抗原比在亲缘关系较近的物种中少。
