Harel J, Bélanger M, Forget C, Jacques M
GREMIP, Département de Pathologie et Microbiologie, Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
Can J Vet Res. 1994 Oct;58(4):302-5.
This study was undertaken to assess the discriminatory value of restriction endonuclease fingerprinting (REF) analysis and ribotyping of 21 Serpulina hyodysenteriae isolates of serotypes 8 and 9. For REF analysis, DNAs were digested with the BglII restriction enzyme and the resultant fragments were separated by polyacrylamide gel electrophoresis. For ribotyping, hybridization of BglII genomic fragments with a probe of rrnB operon using an Escherichia coli rDNA probe was performed on all isolates. Although many isolates shared a common pattern by BglII REF and BglII ribotyping analysis, differences among some S. hyodysenteriae isolates were observed. REF and ribotyping using BglII restriction enzyme, were not specific for serotypes. The predominance of an REF and a ribotype pattern among S. hyodysenteriae isolates from Quebec suggested that epidemiologically important S. hyodysenteriae types occur in different swine herds.
本研究旨在评估限制性内切酶指纹图谱（REF）分析和核糖体分型对21株8型和9型猪痢疾蛇形螺旋体分离株的鉴别价值。对于REF分析，用BglII限制性内切酶消化DNA，所得片段通过聚丙烯酰胺凝胶电泳分离。对于核糖体分型，使用大肠杆菌rDNA探针，对所有分离株进行BglII基因组片段与rrnB操纵子探针的杂交。尽管通过BglII REF和BglII核糖体分型分析，许多分离株具有共同模式，但仍观察到一些猪痢疾蛇形螺旋体分离株之间存在差异。使用BglII限制性内切酶的REF和核糖体分型对血清型不具有特异性。来自魁北克的猪痢疾蛇形螺旋体分离株中，一种REF和一种核糖体分型模式占主导地位，这表明在不同猪群中存在具有重要流行病学意义的猪痢疾蛇形螺旋体类型。
