Wang J, Pepinsky B, Karpusas M, Liu J, Osborn L
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138.
Proteins. 1994 Nov;20(3):287-90. doi: 10.1002/prot.340200310.
A molecular fragment comprising the first two domains of the human vascular cell adhesion molecule-1 (VCAM-1) has been crystallized by the vapor diffusion method. Two crystal forms have been examined by X-ray analysis: One crystal form belongs to the space group C2 with two molecules in the asymmetric unit and cell parameters: a = 122.1 A, b = 48.9 A, c = 73.4 A, and beta = 117.4 degrees. The other crystal form belongs to the space group P2(1) with one molecule in the asymmetric unit and cell parameters: a = 40.4 A, b = 45.7 A, c = 54.7 A, and beta = 100.5 degrees. Diffraction data up to 1.9 A resolution have been collected for the C2 crystal form.
包含人血管细胞黏附分子-1（VCAM-1）前两个结构域的分子片段已通过气相扩散法结晶。通过X射线分析研究了两种晶体形式：一种晶体形式属于空间群C2，不对称单元中有两个分子，晶胞参数为：a = 122.1 Å，b = 48.9 Å，c = 73.4 Å，β = 117.4°。另一种晶体形式属于空间群P2(1)，不对称单元中有一个分子，晶胞参数为：a = 40.4 Å，b = 45.7 Å，c = 54.7 Å，β = 100.5°。已收集到C2晶体形式分辨率高达1.9 Å的衍射数据。
