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使用非放射性原位杂交进行RNA检测。

RNA detection using non-radioactive in situ hybridization.

作者信息

Wilkinson D G

机构信息

Laboratory of Developmental Neurobiology, National Institute for Medical Research, Mill Hill, London, UK.

出版信息

Curr Opin Biotechnol. 1995 Feb;6(1):20-3. doi: 10.1016/0958-1669(95)80004-2.

DOI:10.1016/0958-1669(95)80004-2
PMID:7534503
Abstract

Methods of non-radioactive in situ hybridization to RNA now enable the simultaneous detection of two RNAs in the same tissue. Sensitivity has been increased by several modifications, including the use of the polymerase chain reaction. Recently, in situ hybridization has been combined with lineage tracing to determine the origin of cells expressing a specific gene.

摘要

非放射性RNA原位杂交方法现在能够在同一组织中同时检测两种RNA。通过包括使用聚合酶链反应在内的多种改进，灵敏度得到了提高。最近，原位杂交已与谱系追踪相结合，以确定表达特定基因的细胞的起源。

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