Stevenson A, Yaqoob M, Mason H, Pai P, Bell G M
Biomedical Sciences Group, Health and Safety Laboratory, Sheffield, UK.
QJM. 1995 Jan;88(1):23-8.
To investigate possible mechanisms of hydrocarbon or solvent-induced renal damage, we studied three groups of healthy men employed in a UK manufacturing plant. Group 1 (n = 111) were occupationally exposed to hydrocarbon-based paints, Group 2 (n = 100) were occupationally exposed to petroleum-based mineral oils, and Group 3 (n = 92) had low background occupational exposure to hydrocarbons. Occupational atmospheric exposure levels for toluene, xylene, butanol and oil mist around the time of this study were within UK permissible limits. Group 4 (controls) were males with no known occupational hydrocarbon or solvent exposure (n = 108). Circulating laminin antibodies and the auto-antibody implicated in Goodpasture's syndrome (anti-GBM) were measured, as were serum laminin, a basement membrane turnover marker, and soluble E-selectin, an endothelial activation marker. Group 1 had a significantly greater proportion of subjects with high levels of both anti-laminin antibodies and soluble E-selectin; Group 2 had significantly more subjects with raised anti-GBM antibodies, laminin and soluble E-selectin. Mean levels of soluble E-selectin were increased in Groups 1 and 2. In a small but significant proportion of these workers exposed to hydrocarbons/mixed solvents there are alterations both to basement membranes, resulting in auto-antibody production, and to overlying vascular endothelial cells.
为了研究碳氢化合物或溶剂引起肾损伤的可能机制，我们对英国一家制造工厂的三组健康男性进行了研究。第一组（n = 111）职业性接触烃基涂料，第二组（n = 100）职业性接触石油基矿物油，第三组（n = 92）职业背景下对碳氢化合物的接触水平较低。在本研究期间，甲苯、二甲苯、丁醇和油雾的职业大气暴露水平在英国允许限值范围内。第四组（对照组）为无已知职业性碳氢化合物或溶剂接触史的男性（n = 108）。检测了循环层粘连蛋白抗体以及与肺出血肾炎综合征相关的自身抗体（抗肾小球基底膜抗体），还检测了血清层粘连蛋白（一种基底膜更新标志物）和可溶性E选择素（一种内皮激活标志物）。第一组中抗层粘连蛋白抗体和可溶性E选择素水平高的受试者比例显著更高；第二组中抗肾小球基底膜抗体、层粘连蛋白和可溶性E选择素升高的受试者明显更多。第一组和第二组中可溶性E选择素的平均水平升高。在这些接触碳氢化合物/混合溶剂的工人中，有一小部分但比例显著的人，其基底膜发生改变，导致自身抗体产生，同时覆盖其上的血管内皮细胞也发生改变。
