来自大肠杆菌O113脂多糖的O-特异性多糖的结构

The structure of the O-specific polysaccharide from Escherichia coli O113 lipopolysaccharide.

作者信息

Parolis H, Parolis L A

机构信息

School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa.

出版信息

Carbohydr Res. 1995 Feb 17;267(2):263-9. doi: 10.1016/0008-6215(94)00303-w.

DOI:10.1016/0008-6215(94)00303-w

PMID:7534644

Abstract

The O-specific polysaccharide from Escherichia coli O113 lipopolysaccharide was separated from the core and lipid A by mild acid hydrolysis and purified by GPC. Methylation analysis and 1H and 13C NMR spectroscopic studies of the O-deacetylated polysaccharide allowed the determination of the structure of the pentasaccharide repeating unit of the polysaccharide which can be written as [equation: see text] The position of the O-acetyl groups was not determined.

摘要

通过温和酸水解从大肠杆菌O113脂多糖中分离出O-特异性多糖，并使其与核心多糖和脂质A分离，然后通过凝胶渗透色谱法（GPC）进行纯化。对O-脱乙酰化多糖进行甲基化分析以及1H和13C核磁共振光谱研究，从而确定该多糖五糖重复单元的结构，其可表示为[方程式：见正文]。O-乙酰基的位置未确定。