Dun N J, Dun S L, Wu S Y, Förstermann U
Department of Anatomy, Medical College of Ohio, Toledo 43614.
Neurosci Lett. 1993 Aug 6;158(1):51-4. doi: 10.1016/0304-3940(93)90610-w.
Nitric oxide synthase-immunoreactivity (NOS-IR) was detected in strands of nerve fibers entering the rat superior cervical ganglia (SCG) and in nerve fibers forming a plexus beneath the capsule of adrenal glands. Within the SCG, varicose NOS-IR fibers encircled virtually all postganglionic neurons and small diameter cells, presumably small intensely fluorescent (SIF) cells. Perikarya of SIF cells exhibited strong NOS-IR, whereas the level appeared to be low in postganglionic neurons. Decentralization of the SCG for 4-6 days markedly reduced the number as well as the intensity of NOS-IR fibers without causing a detectable change of NOS-IR in the postganglionic neurons and SIF cells. Beneath the adrenal capsule, bundles of NOS-IR fibers bifurcated and made a sharp turn to reach the adrenal medulla. Chromaffin cells, which themselves exhibited fairly strong NOS-IR, appeared to be surrounded by NOS-IR fibers. The result shows that NOS-IR is present in pre- and post-synaptic elements of the sympathetic ganglia and adrenal medulla, representing a complex system that may regulate the activity of ganglionic neurons and chromaffin cells via a number of sites of action.
在进入大鼠颈上神经节（SCG）的神经纤维束以及肾上腺被膜下形成神经丛的神经纤维中检测到一氧化氮合酶免疫反应性（NOS-IR）。在SCG内，曲张的NOS-IR纤维几乎环绕着所有节后神经元和小直径细胞，推测为小而强荧光（SIF）细胞。SIF细胞的胞体呈现强烈的NOS-IR，而节后神经元中的水平似乎较低。SCG去神经支配4 - 6天显著减少了NOS-IR纤维的数量和强度，而未引起节后神经元和SIF细胞中NOS-IR的可检测变化。在肾上腺被膜下，NOS-IR纤维束分支并急转弯到达肾上腺髓质。嗜铬细胞本身表现出相当强的NOS-IR，似乎被NOS-IR纤维所包围。结果表明，NOS-IR存在于交感神经节和肾上腺髓质的突触前和突触后成分中，代表了一个复杂的系统，该系统可能通过多个作用位点调节神经节神经元和嗜铬细胞的活性。
