Kerr J R, O'Neill H J, Deleys R, Wright C, Coyle P V
Regional Virus Laboratory, Royal Victoria Hospital, Belfast, Northern Ireland, UK.
J Immunol Methods. 1995 Mar 13;180(1):101-6. doi: 10.1016/0022-1759(94)00305-g.
Native parvovirus B19 was used as antigen to produce a mouse monoclonal antibody, R92F6, which reacted with B19 VP1 and VP2, neutralised the virus in bone marrow culture, and labelled infected cells in paraffin-embedded tissues from cases of B19-related fetal hydrops. The B19 epitope recognised by R92F6 (amino acids 328-344 from the amino terminal region of B19 VP2) appears to be highly conserved, since these tissue specimens were obtained over a 13 year period from widely spaced locations in the UK. This epitope was synthesised as a peptide (S7b) which was used as antigen to produce a mouse monoclonal antibody, 3H8, which specifically reacted with the B19 capsid proteins VP1 and VP2 in immunofluorescence and immunoblot assays. 3H8 was also capable of labelling formalin-fixed, paraffin-embedded, B19-infected fetal tissue and was shown to be of the same isotype as R92F6 (IgG1). Highly conserved epitopes derived from conserved amino acid sequences are valuable in the diagnosis of infectious disease. If these can be recognised and accurately synthesised, the production of specific mouse monoclonal antibodies may be possible for many human pathogens. Considering the vast amount of sequence data available in the literature, this approach seems to be both feasible and of wide potential.
天然细小病毒B19被用作抗原，以产生一种小鼠单克隆抗体R92F6，该抗体与B19 VP1和VP2发生反应，在骨髓培养中中和病毒，并标记来自B19相关胎儿水肿病例的石蜡包埋组织中的感染细胞。R92F6识别的B19表位（来自B19 VP2氨基末端区域的氨基酸328 - 344）似乎高度保守，因为这些组织标本是在13年期间从英国相距甚远的地点获得的。该表位被合成为一种肽（S7b），用作抗原以产生一种小鼠单克隆抗体3H8，该抗体在免疫荧光和免疫印迹试验中与B19衣壳蛋白VP1和VP2特异性反应。3H8也能够标记福尔马林固定、石蜡包埋的B19感染胎儿组织，并且显示与R92F6具有相同的同种型（IgG1）。源自保守氨基酸序列的高度保守表位在传染病诊断中很有价值。如果这些表位能够被识别并准确合成，那么对于许多人类病原体来说，生产特异性小鼠单克隆抗体可能是可行的。考虑到文献中可用的大量序列数据，这种方法似乎既可行又具有广泛的潜力。
