Morey A L, O'Neill H J, Coyle P V, Fleming K A
University of Oxford, Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, U.K.
J Pathol. 1992 Feb;166(2):105-8. doi: 10.1002/path.1711660204.
Human parvovirus B19 is a cause of aplastic crises in patients with haemolytic anaemias, prolonged bone marrow failure in the immunosuppressed, and fetal death secondary to non-immune hydrops. The immunohistological detection of parvovirus B19 in formalin-fixed, paraffin-embedded tissues has not previously been reported, and definitive diagnosis of infection in such specimens has relied on the use of specialized DNA hybridization and amplification techniques. A new monoclonal antibody to B19 capsid proteins, R92F6, was found to be capable of labelling infected cells in paraffin-embedded tissues from all 19 cases of parvovirus-related fetal hydrops tested, and in bone marrow from a child with congenital immunodeficiency and chronic parvovirus infection. Viral antigen was detected both in cytoplasmic and in nuclear distributions using the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique without preceding proteolytic digestion. The viral epitope recognized appears to be highly conserved, as specimens were obtained over a 13-year period from widely spaced locations in the U.K. Antibody R92F6 should facilitate rapid diagnosis of parvovirus B19 infection in routinely processed and archival specimens.
人细小病毒B19是导致溶血性贫血患者再生障碍危象、免疫抑制患者长期骨髓衰竭以及非免疫性水肿继发胎儿死亡的病因。此前尚未有在福尔马林固定、石蜡包埋组织中进行细小病毒B19免疫组织学检测的报道，此类标本中感染的确诊依赖于使用专门的DNA杂交和扩增技术。一种针对B19衣壳蛋白的新型单克隆抗体R92F6，在检测的19例与细小病毒相关的胎儿水肿病例的石蜡包埋组织以及一名患有先天性免疫缺陷和慢性细小病毒感染儿童的骨髓中，均能标记受感染细胞。使用碱性磷酸酶抗碱性磷酸酶（APAAP）技术，无需预先进行蛋白水解消化，即可在细胞质和细胞核分布中检测到病毒抗原。由于标本是在13年期间从英国相距甚远的地点获取的，所识别的病毒表位似乎高度保守。抗体R92F6应有助于在常规处理和存档标本中快速诊断细小病毒B19感染。
