Huang W Y, Coltrera M, Schubert M, Morton T, Truelove E
Department of Oral Medicine, School of Dentistry, University of Washington, Seattle.
Oral Surg Oral Med Oral Pathol. 1994 Dec;78(6):748-54. doi: 10.1016/0030-4220(94)90091-4.
As the therapeutic options for malignant lesions expand, early accurate diagnosis of premalignancy is becoming increasingly important in the concept of cancer prevention. Because it has been hypothesized that abnormal cell proliferation is related to subsequent malignant transformation, many proliferation markers such as proliferating cell nuclear antigen have been studied in a variety of malignant tumors. In oral surface epithelium, proliferating cell nuclear antigen activity is restricted to basal layers of normal squamous mucosa. In this preliminary study, 169 formalin-fixed, paraffin-embedded oral epithelial lesions, including 28 carcinomas in situ, 82 epithelial dysplasias, 21 epithelial atypia, and 38 typical epithelial hyperplasias, were studied with a monoclonal antibody, PC10, to determine whether proliferating cell nuclear antigen suprabasal expression correlated with premalignancy. The findings revealed that with progression of lesions toward malignancy, there was a significant predilection for basal/suprabasal staining pattern for proliferating cell nuclear antigen as compared with the strictly basal staining pattern seen in normal and benign epithelial conditions. One unexpected staining pattern, suprabasal positive stain only, was also noted mostly in reactive hyperplasia and dysplasia. The data suggested that a positive basal/suprabasal staining pattern for proliferating cell nuclear antigen is indicative of premalignancy in oral epithelial lesions.
随着恶性病变治疗选择的不断扩展，在癌症预防概念中，癌前病变的早期准确诊断变得越来越重要。由于据推测异常细胞增殖与随后的恶性转化有关，许多增殖标志物如增殖细胞核抗原已在多种恶性肿瘤中进行了研究。在口腔表面上皮中，增殖细胞核抗原活性仅限于正常鳞状黏膜的基底层。在这项初步研究中，使用单克隆抗体PC10对169例福尔马林固定、石蜡包埋的口腔上皮病变进行了研究，这些病变包括28例原位癌、82例上皮发育异常、21例上皮异型增生和38例典型上皮增生，以确定增殖细胞核抗原的基底层以上表达是否与癌前病变相关。研究结果显示，随着病变向恶性进展，与正常和良性上皮情况下所见的严格基底层染色模式相比，增殖细胞核抗原的基底层/基底层以上染色模式有明显的偏好。还发现一种意外的染色模式，即仅基底层以上阳性染色，大多出现在反应性增生和发育异常中。数据表明，增殖细胞核抗原的基底层/基底层以上阳性染色模式表明口腔上皮病变存在癌前病变。
