Sugii S
Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
J Vet Med Sci. 1994 Dec;56(6):1047-50. doi: 10.1292/jvms.56.1047.
To identify the antigenic determinants of Clostridium perfringens enterotoxin (CPE), overlapping peptides corresponding to the entire amino acid sequence of CPE were synthesized and their antigenicity and immunogenicity were analyzed. Of the 21 synthetic peptides (C-1 to C-21) tested, peptide C-7 (amino acid residues 91-110) showed the highest reactivity with rabbit antibodies against CPE. Peptides C-4 (46-65), C-9 (121-140), and C-11 (151-170) were reactive with the antibodies, whereas peptides C-2 (16-35), C-3 (31-50), and C-20 (286-305) were less reactive. Other peptides were much less reactive. On the other hand, all of the synthetic peptides reactive with the anti-CPE antibodies were found to elicit rabbit antibodies reactive with both their respective antigens and native CPE molecule. These findings demonstrate that CPE molecule contains at least 10 different antigenic determinants which are all immunogenic and supposed to be responsible for functionally and biologically active sites.
为鉴定产气荚膜梭菌肠毒素（CPE）的抗原决定簇，合成了与CPE完整氨基酸序列相对应的重叠肽，并分析了它们的抗原性和免疫原性。在测试的21种合成肽（C-1至C-21）中，肽C-7（氨基酸残基91 - 110）与抗CPE兔抗体表现出最高反应性。肽C-4（46 - 65）、C-9（121 - 140）和C-11（151 - 170）与抗体有反应，而肽C-2（16 - 35）、C-3（31 - 50）和C-20（286 - 305）反应性较低。其他肽的反应性更低。另一方面，所有与抗CPE抗体反应的合成肽都能诱导出与各自抗原和天然CPE分子均有反应的兔抗体。这些发现表明，CPE分子至少包含10种不同的抗原决定簇，它们均具有免疫原性，并且被认为与功能和生物活性位点有关。
