使用不同合成肽对葡萄球菌肠毒素A进行表位分析。
Epitope analysis of staphylococcal enterotoxin A using different synthetic peptides.
作者信息
Hu D L, Imai A, Ono K, Sasaki S, Nakane A, Sugii S, Shinagawa K
机构信息
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
出版信息
J Vet Med Sci. 1998 Sep;60(9):993-6. doi: 10.1292/jvms.60.993.
The antigenic determinants (or epitopes) of staphylococcal enterotoxin A (SEA) were analyzed using synthetic peptides and rabbit antibodies to their corresponding peptides. Of 12 different synthetic peptides tested, peptides A-1 (corresponding to the amino acid sequence 1-20), A-5 (81-100), and A-8 (141-160) were reactive with anti-SEA antibodies. However, all synthetic peptides were found to elicit antibodies reactive with native SEA molecule. These findings suggest that native SEA molecule contains at least 3 different antigenic determinants.
利用合成肽和针对相应肽的兔抗体分析了葡萄球菌肠毒素A（SEA）的抗原决定簇（或表位）。在所测试的12种不同合成肽中，肽A-1（对应于氨基酸序列1-20）、A-5（81-100）和A-8（141-160）与抗SEA抗体发生反应。然而，发现所有合成肽均能引发与天然SEA分子发生反应的抗体。这些发现表明天然SEA分子至少包含3种不同的抗原决定簇。
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