绵羊小胶质细胞的凝集素染色。
Lectin staining of sheep microglia.
作者信息
Pennell N A, Hurley S D, Streit W J
机构信息
Department of Neuroscience, JHMHC, University of Florida, Gainesville 32610.
出版信息
Histochemistry. 1994 Dec;102(6):483-6. doi: 10.1007/BF00269580.
The B4-isolectin from Griffonia simplicifolia is known to stain microglial cells in a variety of species. The present report describes a lectin staining method that has been modified to facilitate staining of resting microglia, as well as perivascular cells, in vibratome sections of normal sheep brain. This modified method employs tissue fixed in formaldehyde or paraformaldehyde and requires incubating sections with Triton X-100 prior to staining.
已知来自西非豆科植物的B4异凝集素可对多种物种的小胶质细胞进行染色。本报告描述了一种凝集素染色方法，该方法经过改进，以便于在正常绵羊脑振动切片中对静息小胶质细胞以及血管周围细胞进行染色。这种改进方法使用甲醛或多聚甲醛固定的组织，并且在染色前需要用Triton X-100孵育切片。
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