Ede A J, Crawford A M
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Anim Genet. 1995 Feb;26(1):43-4. doi: 10.1111/j.1365-2052.1995.tb02619.x.
The microsatellite described for the glycine- and tyrosine-rich keratin locus (KAP8) in sheep was used to type nine large three-generation families comprising the AgResearch International Mapping Flock. The apparent non-Mendelian inheritance in these families suggested that some of the microsatellite alleles were not being amplified. Sequence analysis showed that a deletion within one of the published primer sites was the likely cause of the 'null' alleles. By redesigning the primer all alleles could be amplified.
用于绵羊富含甘氨酸和酪氨酸的角蛋白基因座（KAP8）的微卫星被用于对由AgResearch国际定位羊群组成的9个大型三代家系进行分型。这些家系中明显的非孟德尔遗传表明一些微卫星等位基因未被扩增。序列分析表明，已发表的引物位点之一内的缺失可能是“无效”等位基因的原因。通过重新设计引物，所有等位基因都可以被扩增。
