Matsuo S, Toyokuni S, Osaka M, Hamazaki S, Sugiyama T
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Biochem Biophys Res Commun. 1995 Mar 28;208(3):1021-7. doi: 10.1006/bbrc.1995.1436.
Recently, DNA of extinct creatures have been brought into analyses. The difficulty in this field of research is DNA degradation. Oxidative damage is considered to be one of the causes of the DNA degradation. We studied how DNA in dried tissue was affected by atmospheric oxygen using freeze-dried rat liver as a model. In tissues exposed to oxygen, DNA degradation occurred within several months and the amount of 8-hydroxy-2'-deoxyguanosine in DNA rapidly increased. The DNA degradation was inhibited by lipid extraction prior to the exposure to atmospheric oxygen. Purified lambda phage DNA was not affected by oxygen. Cellular DNA and RNA were degraded slowly in nitrogen air. These results suggest that both atmospheric and endogenous oxygens play a role in DNA degradation in dried tissues.
最近，已对灭绝生物的DNA进行分析。该研究领域的困难在于DNA降解。氧化损伤被认为是DNA降解的原因之一。我们以冻干大鼠肝脏为模型，研究了干燥组织中的DNA如何受到大气氧的影响。在暴露于氧气的组织中，DNA降解在几个月内就会发生，并且DNA中8-羟基-2'-脱氧鸟苷的量迅速增加。在暴露于大气氧之前进行脂质提取可抑制DNA降解。纯化的λ噬菌体DNA不受氧气影响。细胞DNA和RNA在氮气中降解缓慢。这些结果表明，大气氧和内源性氧在干燥组织中的DNA降解中均起作用。
