Ding H, Wang J, Dorsey J G, Caruso J A
Department of Chemistry, University of Cincinnati, OH 45221-0172.
J Chromatogr A. 1995 Mar 10;694(2):425-31. doi: 10.1016/0021-9673(94)01085-s.
Four environmentally and biologically important arsenic species, dimethylarsenic acid (DMA), monomethylarsonic acid (MMA), As(III) and As(V) are separated by micellar liquid chromatography. Linear dynamic ranges for the four species are three orders of magnitude and detection limits are in the picogram range with inductively coupled plasma mass spectrometric (ICP-MS) detection. This paper discussed in detail the development of the chromatographic conditions. The micellar mobile phase, which consisted of 0.05 M cetyltrimethylammonium bromide, 10% propanol and 0.02 M borate buffer, showed good compatibility with ICP-MS. This method allowed direct injection of urine samples onto the chromatographic system without extensive pretreatment and presented no interference from chlorine in the matrix. Detection limits are comparable with other LC-ICP-MS studies. An SRM urine sample was used to demonstrate the applicability of this technique to "real-life" situations. Results indicated that DMA, MMA and As(V) were present in the urine sample.
四种对环境和生物具有重要意义的砷物种，即二甲基砷酸（DMA）、一甲基砷酸（MMA）、As(III) 和 As(V)，通过胶束液相色谱法进行分离。这四种物种的线性动态范围为三个数量级，采用电感耦合等离子体质谱（ICP-MS）检测时，检测限在皮克范围内。本文详细讨论了色谱条件的优化。由0.05 M十六烷基三甲基溴化铵、10%丙醇和0.02 M硼酸盐缓冲液组成的胶束流动相，与ICP-MS具有良好的兼容性。该方法允许在无需大量预处理的情况下将尿液样品直接注入色谱系统，且基质中的氯不会产生干扰。检测限与其他液相色谱-电感耦合等离子体质谱研究相当。使用一个标准参考物质尿液样品来证明该技术在“实际”情况下的适用性。结果表明，尿液样品中存在DMA、MMA和As(V)。
