Rouhbakhsh D, Moran N A, Baumann L, Voegtlin D J, Baumann P
Microbiology Section, University of California, Davis 95616-8665.
Insect Mol Biol. 1994 Nov;3(4):213-7. doi: 10.1111/j.1365-2583.1994.tb00169.x.
Members of the genus Buchnera constitute a distinct prokaryotic lineage containing the primary endosymbionts of aphids (Homoptera: Aphidoidea). Using synthetic oligonucleotides in conjunction with the polymerase chain reaction, we propose three approaches for the identification of members of this genus. The first is based on unique sequences within rrs (gene coding for 16S ribosomal RNA). The second is based on a different and unique organization of the ribosomal RNA operons of Buchnera and the close proximity of aroE upstream of rrl (gene coding for 23S rRNA). The third is based on the linkage relationship of argS which is upstream of rrs. Validation of these three approaches requires their more extensive application.
布赫纳氏菌属的成员构成了一个独特的原核生物谱系，其中包含蚜虫（同翅目：蚜总科）的主要内共生菌。我们使用合成寡核苷酸结合聚合酶链反应，提出了三种鉴定该属成员的方法。第一种方法基于rrs（编码16S核糖体RNA的基因）内的独特序列。第二种方法基于布赫纳氏菌核糖体RNA操纵子的不同且独特的组织方式，以及aroE在rrl（编码23S rRNA的基因）上游的紧密相邻位置。第三种方法基于rrs上游的argS的连锁关系。这三种方法的验证需要更广泛的应用。
