Characterization of a putative 23S-5S rRNA operon of Buchnera aphidicola (endosymbiont of aphids) unlinked to the 16S rRNA-encoding gene.

Buchnera aphidicola (Ba) is an endosymbiont of the aphid Schizaphis graminum. In order to obtain information on highly expressed genes, we have chosen to study Ba genes coding for rRNAs. Previously, the single-copy rrs gene was cloned and sequenced [Munson et al., Gene 137 (1993) 171-178], and found to constitute a single transcription unit unlinked to rrl and rrf. In the present study, a 6.1-kb Ba DNA fragment containing rrl was cloned into Escherichia coli (Ec) and sequenced. Based on sequence similarity to Ec, the following genes were identified: aroE-tRNA(Glu)-rrl-rrf-cysS. AroE and CysS had 48 and 54% amino acid (aa) identity, respectively, to the corresponding Ec proteins; tRNA(Glu), rrl and rrf had 80-90% nucleotide (nt) identity with the corresponding genes of Ec rrnB. Ba tRNA(Glu)-rrl-rrs appears to be part of a single transcriptional unit; a putative promoter and a Rho-independent terminator were identified. Comparisons of sequences of aroE-rrl from endosymbionts of seven additional species of aphids indicated conservation of the -35 (TTGACT) and -10 (TGTAA/TT) promoter regions, and boxA, tRNA(Glu) and boxC. Secondary structure analysis indicated that the Ba tRNA(Glu)-rrl-rrf operon resembled the homologous region of Ec rrnB. The results of this and previous studies indicate that Ba differs from most bacteria in having the single-copy rRNA genes organized into two transcription units.