Rozee K R, Haase D, Macdonald N E, Johnson W M
Department of Microbiology, Victoria General Hospital, Dalhousie University, Halifax, Nova Scotia, Canada.
Diagn Microbiol Infect Dis. 1994 Dec;20(4):181-6. doi: 10.1016/0732-8893(94)90001-9.
Multiple isolates of Pseudomonas cepacia, from two cystic fibrosis (CF) patients who were chronically infected and two others who suffered acute fatal lung infections, were examined by multilocus enzyme electrophoresis and four-enzyme ribotyping. The strains isolated from the fatalities belonged to a clone, electropherotype 12 (ET12) that is endemic in the Ontario patients' province of origin. ET12 strains have also been isolated from outbreaks in CF patients in the United Kingdom, where they are considered to be strains of high virulence and transmissibility and epidemiologically related to Ontario strains. Four-enzyme ribotyping (EcoRI, Xho, PstI, and ClaI) established the close genetic relationship of the Ontario ET12 isolates and those from the United Kingdom, particularly an isolate from Manchester. In addition, four enzyme ribotypes of the sequential isolates taken during life and at autopsy of the ET12 clone were highly variable in comparison with the stability of the ribotypes of clone ET16 isolated sequentially from living chronic carriers. This extreme ribotype variability may be indicative of a highly virulent strain and poor prognosis. Isolates from our chronically infected CF patients belonged to a different clone, ET16, and it is also endemic in its region, 1000 miles east of ET12, in Nova Scotia. In both endemic circumstances, person-to-person transmission was easily demonstrated by four-enzyme ribotyping. The ET12 clone was found to be transmitted among summer campers and during a nosocomial outbreak, whereas an E16 strain was found to infect a sibling of a chronically infected patient; both infections were of the same ribotype.
对来自两名慢性感染的囊性纤维化（CF）患者以及另外两名遭受急性致命肺部感染患者的多个洋葱伯克霍尔德菌分离株进行了多位点酶电泳和四酶核糖体分型检测。从死亡患者身上分离出的菌株属于一个克隆，即电泳型12（ET12），该克隆在安大略省患者的原籍省份呈地方性流行。ET12菌株也曾从英国CF患者的疫情中分离出来，在英国它们被认为是高毒力和高传播性的菌株，并且在流行病学上与安大略省的菌株相关。四酶核糖体分型（EcoRI、Xho、PstI和ClaI）确定了安大略省ET12分离株与来自英国的分离株，特别是来自曼彻斯特的一株分离株之间的密切遗传关系。此外，与从慢性携带者活体中依次分离出的ET16克隆的核糖体分型稳定性相比，在ET12克隆患者生前和尸检时依次采集的分离株的四种酶核糖体分型高度可变。这种极端的核糖体分型变异性可能表明菌株毒力高且预后不良。我们慢性感染的CF患者的分离株属于另一个克隆，即ET16，它在其所在地区（位于ET12以东1,000英里的新斯科舍省）也呈地方性流行。在这两种地方性流行情况下，通过四酶核糖体分型很容易证明人与人之间的传播。发现ET12克隆在夏令营参与者之间传播以及在一次医院感染暴发期间传播，而发现一株E16菌株感染了一名慢性感染患者的兄弟姐妹；两次感染的核糖体分型相同。
