Pitt T L, Kaufmann M E, Patel P S, Benge L C, Gaskin S, Livermore D M
Laboratory of Hospital Infection, Central Public Health Laboratory, London, UK.
J Med Microbiol. 1996 Mar;44(3):203-10. doi: 10.1099/00222615-44-3-203.
The spread of Burkholderia cepacia among cystic fibrosis (CF) patients in the UK prompted an investigation into whether an epidemic strain was responsible. A total of 366 B. cepacia isolates from 178 CF patients in 17 centres was examined by ribotyping and pulsed-field gel electrophoresis (PFGE). Associations were also sought between antibiotic resistance and strain type. More than 50 ribotype patterns were found but one, termed ribotype 1, was identified from 68 patients in eight centres. One centre had a single patient with this type while, in others, most or all patients harboured this organism. Small clusters of apparent cross-colonisation within centres were also evident for some other ribotypes. PFGE confirmed that ribotype 1 isolates were genetically similar. Ribotype 1 isolates were not markedly more resistant to antimicrobial agents than were other isolates, and the MICs of individual antibiotics were no more tightly clustered for ribotype 1 isolates than for others. Most isolates were resistant to ciprofloxacin, amikacin, gentamicin, tobramycin, carbenicillin, cefuroxime, cefotaxime, imipenem, biapenem, chloramphenicol, tetracycline, trimethoprim and sulphamethoxazole, but > or = 77% were susceptible to ceftazidime, piperacillin, piperacillin/ tazobactam and meropenem. We conclude that numerous strains of B. cepacia colonise CF patients in the UK and Ireland but that one epidemic strain has spread in at least eight centres. Isolates of this strain appear homogenous in total genomic profile but very variable in antibiotic susceptibility.
洋葱伯克霍尔德菌在英国囊性纤维化（CF）患者中的传播促使人们调查是否存在一种流行菌株。对来自17个中心的178例CF患者的366株洋葱伯克霍尔德菌分离株进行了核糖体分型和脉冲场凝胶电泳（PFGE）检测。同时还研究了抗生素耐药性与菌株类型之间的关联。发现了50多种核糖体分型模式，但有一种被称为核糖体分型1的模式，在8个中心的68例患者中被鉴定出来。一个中心只有一名患者感染这种类型，而在其他中心，大多数或所有患者都携带这种病菌。对于其他一些核糖体分型，中心内明显的交叉定植小簇也很明显。PFGE证实核糖体分型1的分离株在基因上相似。核糖体分型1的分离株对抗菌药物的耐药性并不比其他分离株明显更强，而且与其他分离株相比，核糖体分型1分离株的个别抗生素最低抑菌浓度（MIC）并没有更紧密地聚集在一起。大多数分离株对环丙沙星、阿米卡星、庆大霉素、妥布霉素、羧苄青霉素、头孢呋辛、头孢噻肟、亚胺培南、比阿培南、氯霉素、四环素、甲氧苄啶和磺胺甲恶唑耐药，但≥77%的分离株对头孢他啶、哌拉西林、哌拉西林/他唑巴坦和美罗培南敏感。我们得出结论，在英国和爱尔兰，有许多株洋葱伯克霍尔德菌定植于CF患者，但有一种流行菌株至少在8个中心传播。该菌株的分离株在全基因组图谱上似乎是同质的，但在抗生素敏感性方面差异很大。
