Takaishi T, Morita Y, Hirai K, Yamaguchi M, Ohta K, Noda E, Morita T, Ito K, Miyamoto T
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Allergy. 1994 Dec;49(10):837-42. doi: 10.1111/j.1398-9995.1994.tb00784.x.
To evaluate the contribution of human lung mast cells (HLMC) to allergic inflammation, we investigated whether or not cytokines, including stem-cell factor (SCF), monocyte chemotactic and activating factor (MCAF), and RANTES, activate HLMC. SCF induced histamine release from dispersed HLMC in a dose-dependent fashion (P < 0.01). The release was 7.8 +/- 1.0% at 500 ng/ml SCF (n = 9). This response was also observed in chopped lung tissue. HLMC from which surface IgE molecules had been removed by treatment with lactic acid responded to SCF, while these cells lost their response to anti-IgE. The process was relatively rapid and reached a maximum in 5 min. This response required extracellular calcium, and it was observed at 37 degrees C, but not at 4 degrees C or 20 degrees. A brief preincubation (10 min) with lower concentrations of SCF, which were ineffective in releasing histamine, enhanced anti-IgE-induced histamine release (P < 0.05), while its enhancing effect was lost by the longer preincubation (30 min). SCF did not prime basophils to enhance stimulated-histamine release. Interleukin (IL)-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, granulocyte/macrophage-colony stimulating factor (GM-CSF), MCAF, and RANTES neither induced histamine release nor enhanced the release stimulated by anti-IgE after a 10- or 30-min preincubation. The combination of IL-3 and IL-4 showed no effect on histamine release from HLMC. Leukotriene (LT)C4/D4/E4 production by SCF was negligible, as compared with anti-IgE-induced LT production.(ABSTRACT TRUNCATED AT 250 WORDS)
为评估人肺肥大细胞（HLMC）在变应性炎症中的作用，我们研究了包括干细胞因子（SCF）、单核细胞趋化激活因子（MCAF）和调节激活正常T细胞表达和分泌因子（RANTES）在内的细胞因子是否能激活HLMC。SCF以剂量依赖方式诱导分散的HLMC释放组胺（P<0.01）。在500 ng/ml SCF时释放率为7.8±1.0%（n=9）。在切碎的肺组织中也观察到这种反应。用乳酸处理去除表面IgE分子的HLMC对SCF有反应，而这些细胞失去了对抗IgE的反应。该过程相对较快，5分钟达到最大值。这种反应需要细胞外钙，在37℃时可观察到，而在4℃或20℃时未观察到。用较低浓度的SCF进行短暂预孵育（10分钟），其本身不能有效释放组胺，但可增强抗IgE诱导的组胺释放（P<0.05），而较长时间预孵育（30分钟）则会使其增强作用消失。SCF不能使嗜碱性粒细胞致敏以增强刺激组胺释放。白细胞介素（IL）-1α、IL-1β、IL-3、IL-4、IL-5、粒细胞/巨噬细胞集落刺激因子（GM-CSF）、MCAF和RANTES在预孵育10或30分钟后既不诱导组胺释放，也不增强抗IgE刺激的释放。IL-3和IL-4的组合对HLMC组胺释放无影响。与抗IgE诱导的白三烯（LT）生成相比，SCF诱导的LTC4/D4/E生产可忽略不计。（摘要截短于250字）
