Louis R, Tilkin P, Poncelet M, Corhay J L, Mendez P, Weber T, Radermecker M
Department of Pneumology, University Hospital, Sart-Tilman, Liège, Belgium.
Allergy. 1995 Apr;50(4):340-8. doi: 10.1111/j.1398-9995.1995.tb01158.x.
We investigated the effects of stem cell factor (SCF) on histamine release (HR) from human bronchoalveolar lavage (BAL) mast cells. BAL cells were recovered from lavage performed in patients undergoing clinical bronchoscopy. SCF (0.02-20 ng/ml), which is by itself a poor secretagogue (mean +/- SEM HR: 3.7 +/- 0.9%; n = 27), strongly enhanced HR induced by anti-IgE in a concentration-related manner. Significant potentiation began at 0.2 ng/ml (30 +/- 10%; p < 0.05; n = 12) and reached a plateau at 2 ng/ml (40 +/- 10%; P < 0.01 at 2 ng/ml and 45 +/- 10%; P < 0.01 at 20 ng/ml; n = 12). In contrast, SCF failed to enhance HR induced by calcium ionophore A23187. Among the BAL cell samples initially unresponsive to anti-IgE (55% of samples), 36% (10/28) were converted to responders if the cells were shortly preincubated with SCF. In 25% of samples (7/27), SCF (20 ng/ml) caused direct HR of 10 +/- 2.1%. The mast cells which released histamine when challenged with SCF also secreted higher levels of histamine in response to anti-IgE and calcium ionophore than those nonresponsive to SCF. While interleukin (IL)-3 and IL-5 (20 ng/ml) were unable to modulate immunologic HR, GM-CSF (20 ng/ml) produced significant potentiation (P < 0.05), which was, however, smaller than that observed with SCF.(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了干细胞因子（SCF）对人支气管肺泡灌洗（BAL）肥大细胞组胺释放（HR）的影响。BAL细胞取自接受临床支气管镜检查患者的灌洗液。SCF本身（0.02 - 20 ng/ml）是一种较弱的促分泌剂（平均±标准误HR：3.7±0.9%；n = 27），但它能以浓度依赖的方式显著增强抗IgE诱导的HR。在0.2 ng/ml时开始有显著增强作用（30±10%；p < 0.05；n = 12），在2 ng/ml时达到平台期（40±10%；2 ng/ml时P < 0.01，20 ng/ml时为45±10%；P < 0.01；n = 12）。相比之下，SCF不能增强钙离子载体A23187诱导的HR。在最初对抗IgE无反应的BAL细胞样本中（55%的样本），如果细胞先用SCF短暂预孵育，36%（10/28）会转变为有反应者。在25%的样本（7/27）中，SCF（20 ng/ml）可引起10±2.1%的直接HR。受到SCF刺激时释放组胺的肥大细胞，对抗IgE和钙离子载体刺激的组胺分泌水平也高于对SCF无反应的肥大细胞。虽然白细胞介素（IL）-3和IL-5（20 ng/ml）不能调节免疫性HR，但粒细胞巨噬细胞集落刺激因子（GM-CSF，20 ng/ml）可产生显著增强作用（P < 0.05），不过比SCF观察到的增强作用小。（摘要截短于250字）
