Endo S, Ohkusa T, Saito Y, Fujiki K, Okayasu I, Sato C
First Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan.
Cancer. 1995 May 1;75(9):2203-8. doi: 10.1002/1097-0142(19950501)75:9<2203::aid-cncr2820750903>3.0.co;2-6.
Helicobacter pylori (H pylori) infection has been suggested to be a risk factor for gastric carcinogenesis. However, those previous studies have been concerned with advanced cancer cases. To the authors' knowledge, no detailed investigation on the prevalence of H pylori in early stage gastric cancer tissue has been performed. The relationship between early stage gastric cancer and the prevalence of H pylori was studied by a immunohistochemical staining analysis.
Sixty-eight patients who were endoscopically and surgically diagnosed as having early stage gastric cancer were enrolled in this study. All tissue specimens were obtained from patients by endoscopic biopsy, and were classified histopathologically as the intestinal-type of early stage gastric cancer in 34 patients (male-to-female ratio, 28:6; age, 64 +/- 11 years) and the diffuse-type of early stage gastric cancer (male-to-female ratio, 23:11; age, 57 +/- 14 years) in the other 34 patients. The amount of H pylori in tissue samples was graded from 0 (no characteristic bacteria) to 3 (numerous bacteria) using the fluorescent microscopic and an immunohistochemical technique.
Twenty-nine of the 34 cases of the intestinal-type of gastric cancer had H pylori infection, as compared with 11 of the 34 cases of diffuse-type early stage gastric cancer. A significantly higher incidence (85%; P < 0.001) of H pylori infection and, thus, higher grading scores of the number of H pylori were found in the intestinal-type early stage gastric cancer.
These findings suggest that the infection of H pylori may have a crucial relationship to the early stages of carcinogenesis of intestinal-type gastric cancer.
幽门螺杆菌（H pylori）感染被认为是胃癌发生的一个危险因素。然而，以往的研究关注的是晚期癌症病例。据作者所知，尚未对早期胃癌组织中幽门螺杆菌的感染率进行详细调查。本研究通过免疫组织化学染色分析，探讨早期胃癌与幽门螺杆菌感染率之间的关系。
本研究纳入了68例经内镜和手术诊断为早期胃癌的患者。所有组织标本均通过内镜活检从患者身上获取，并进行组织病理学分类，其中34例患者（男女比例为28:6；年龄64±11岁）的早期胃癌为肠型，另外34例患者（男女比例为23:11；年龄57±14岁）的早期胃癌为弥漫型。使用荧光显微镜和免疫组织化学技术，将组织样本中幽门螺杆菌的数量从0（无特征性细菌）到3（大量细菌）进行分级。
34例肠型胃癌患者中有29例感染了幽门螺杆菌，而34例弥漫型早期胃癌患者中有11例感染。在肠型早期胃癌中，幽门螺杆菌感染的发生率显著更高（85%；P<0.001），因此幽门螺杆菌数量的分级得分也更高。
这些发现表明，幽门螺杆菌感染可能与肠型胃癌发生的早期阶段存在关键关系。
