Nobes C D, Hall A
Cancer Research Campaign, University College London, England.
Cell. 1995 Apr 7;81(1):53-62. doi: 10.1016/0092-8674(95)90370-4.
Rho and rac, two members of the ras-related superfamily of small GTPases, regulate the polymerization of actin to produce stress fibers and lamellipodia, respectively. We report here that cdc42, another member of the rho family, triggers the formation of a third type of actin-based structure found at the cell periphery, filopodia. In addition to stress fibers, rho controls the assembly of focal adhesion complexes. We now show that rac and cdc42 also stimulate the assembly of multimolecular focal complexes at the plasma membrane. These complexes, which are associated with lamellipodia and filopodia, contain vinculin, paxillin, and focal adhesion kinase, but are distinct from and formed independently of rho-induced focal adhesions. Activation of cdc42 in Swiss 3T3 cells leads to the sequential activation of rac and then rho, suggesting a molecular model for the coordinated control of cell motility by members of the rho family of GTPases.
Rho和Rac是小GTP酶的ras相关超家族的两个成员，分别调节肌动蛋白的聚合以产生应力纤维和片状伪足。我们在此报告，rho家族的另一个成员cdc42触发了在细胞周边发现的第三种基于肌动蛋白的结构——丝状伪足的形成。除了应力纤维，Rho还控制粘着斑复合物的组装。我们现在表明，Rac和cdc42也刺激质膜上多分子粘着斑复合物的组装。这些与片状伪足和丝状伪足相关的复合物包含纽蛋白、桩蛋白和粘着斑激酶，但与Rho诱导的粘着斑不同且独立形成。在瑞士3T3细胞中激活cdc42会导致Rac的顺序激活，然后是Rho的激活，这提示了一种由GTP酶的rho家族成员协调控制细胞运动的分子模型。
