Font G E, Gauthier D W, Meyer W J, Myles T D, Janda W, Bieniarz A
Department of Obstetrics and Gynecology, University of Illinois, Chicago, USA.
Obstet Gynecol. 1995 May;85(5 Pt 1):656-8. doi: 10.1016/0029-7844(95)00026-n.
To evaluate catalase activity as a rapid predictor of microbial invasion of amniotic fluid (AF).
The study population consisted of 74 patients before 36 weeks' gestation with preterm labor or premature rupture of membranes (PROM). Subjects were excluded if there was evidence of clinical chorioamnionitis or fetal distress at admission. Amniocentesis was done within 24 hours of admission, and the AF was cultured for aerobic and anaerobic bacteria and for Mycoplasma species. All AF samples were Gram stained, and slides were examined by microbiology technologists. Amniotic fluid catalase activity was measured immediately after amniocentesis using a commercially available kit. The sensitivity of the Gram stain and catalase activity were compared using McNemar exact test.
Amniotic fluid cultures were positive in 12 of 37 (32%) patients presenting with preterm labor and in 21 of 37 (56%) patients with PROM. Catalase activity was significantly more sensitive than Gram stain in detecting positive AF cultures in cases of PROM (P < .001) and preterm labor (P < .04).
Catalase activity is a simple, rapid test that is useful in identifying subclinical intra-amniotic infection in patients with preterm labor or PROM.
评估过氧化氢酶活性作为羊水（AF）微生物入侵快速预测指标的价值。
研究对象为74例孕36周前出现早产或胎膜早破（PROM）的患者。若入院时有临床绒毛膜羊膜炎或胎儿窘迫证据则排除该受试者。入院后24小时内进行羊膜腔穿刺，羊水进行需氧菌、厌氧菌及支原体培养。所有羊水样本行革兰染色，玻片由微生物技术人员检查。羊膜腔穿刺后立即使用市售试剂盒测定羊水过氧化氢酶活性。采用McNemar确切概率法比较革兰染色和过氧化氢酶活性的敏感性。
37例早产患者中有12例（32%）羊水培养阳性，37例胎膜早破患者中有21例（56%）羊水培养阳性。在胎膜早破（P <.001）和早产（P <.04）病例中，过氧化氢酶活性在检测羊水培养阳性方面比革兰染色显著更敏感。
过氧化氢酶活性检测是一项简单、快速的检查，有助于识别早产或胎膜早破患者的亚临床羊膜腔内感染。
