Lam K M, Vasconcelos A C
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.
Vet Immunol Immunopathol. 1994 Dec;44(1):45-56. doi: 10.1016/0165-2427(94)90168-6.
Chicken peripheral blood lymphocytes (PBL) were infected with the GB strain of Newcastle disease virus (NDV). At 0, 1.5 and 3 h postinfection, PBL were stained with acridine orange-ethidium bromide and examined for apoptotic and necrotic indices under a UV microscope. At the end of 3 h, the cells were pelleted and were either fixed for electron microscopy or used for DNA extraction. Infected cells showed a significant amount of apoptosis and necrosis when compared with controls. Electron microscopy of the infected cells also showed characteristics of apoptosis, i.e. apoptotic bodies, margination of chromatin with crescent formation and fusion of cells. DNA extracted from virus-infected cells showed extensive fragmentation. Based on these findings it is concluded that NDV, in addition to causing necrosis in chicken lymphocytes, can induce apoptosis.
用新城疫病毒（NDV）GB株感染鸡外周血淋巴细胞（PBL）。在感染后0、1.5和3小时，用吖啶橙 - 溴化乙锭对PBL进行染色，并在紫外显微镜下检查凋亡和坏死指数。在3小时结束时，将细胞沉淀，要么固定用于电子显微镜检查，要么用于DNA提取。与对照相比，感染细胞显示出大量的凋亡和坏死。感染细胞的电子显微镜检查也显示出凋亡的特征，即凋亡小体、染色质边缘化形成新月形以及细胞融合。从病毒感染细胞中提取的DNA显示出广泛的片段化。基于这些发现，可以得出结论，NDV除了导致鸡淋巴细胞坏死外，还可诱导凋亡。
