van Gylswyk N O
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Kungsängen Research Centre, Uppsala.
Int J Syst Bacteriol. 1995 Apr;45(2):297-300. doi: 10.1099/00207713-45-2-297.
A gram-negative, anaerobic, nonmotile, non-spore-forming, rod-shaped bacterium that fermented succinate quantitatively to propionate was isolated from a high dilution of rumen ingesta obtained from a dairy cow fed a production diet containing grass silage as the main roughage source. This organism did not grow on any of the following energy sources: 12 carbohydrates, pyruvate, lactate, 7 dicarboxylic acids, aspartate, citrate, and trans-aconitate. Both rumen fluid and yeast extract were necessary for good growth on succinate. The organism was negative for the following characteristics: production of propionate from threonine, protein digestion, sulfide production, nitrate reduction, catalase activity, and urease activity. There was no growth at 22 degrees C and reduced growth at 45 degrees C compared with growth at 39 degrees C. The DNA base composition was 52 mol% G + C. The complete 16S rRNA sequence (EMBL accession number, X81137) was obtained, and the phylogenetic relationships of the organism were determined. The most closely related genera were the genera Acidaminococcus and Phascolarctobacterium. The name proposed for this bacterium is Succiniclasticum ruminis gen. nov., sp. nov.; the type strain is strain SE10 (= DSM 9236). Additional isolation attempts revealed that S. ruminis is a common inhabitant of the rumina of cows that are fed production diets and of cows on pasture.
从以青贮牧草为主要粗饲料来源的产奶牛瘤胃内容物的高稀释液中分离出一种革兰氏阴性、厌氧、无运动性、不形成芽孢的杆状细菌，该细菌可将琥珀酸定量发酵为丙酸。该微生物不能利用以下任何一种能源生长：12种碳水化合物、丙酮酸、乳酸、7种二羧酸、天冬氨酸、柠檬酸和反乌头酸。瘤胃液和酵母提取物对于该微生物在琥珀酸上的良好生长都是必需的。该微生物具有以下阴性特征：由苏氨酸产生丙酸、蛋白质消化、产生硫化物、硝酸盐还原、过氧化氢酶活性和脲酶活性。与在39℃下生长相比，在22℃下不生长，在45℃下生长减弱。DNA碱基组成为52 mol% G + C。获得了完整的16S rRNA序列（EMBL登录号，X81137），并确定了该微生物的系统发育关系。与其关系最密切的属是氨基酸球菌属和袋熊杆菌属。为该细菌提议的名称是瘤胃琥珀酸分解菌属（Succiniclasticum ruminis），新属，新种；模式菌株是SE10菌株（= DSM 9236）。进一步的分离尝试表明，瘤胃琥珀酸分解菌是采食产奶牛日粮的奶牛和放牧奶牛瘤胃中的常见居民。
