Janssen P H, Liesack W
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
Arch Microbiol. 1995 Jul;164(1):29-35. doi: 10.1007/BF02568731.
Enrichments on succinate plus yeast extract under anoxic conditions from intertidal mud-flat sediments yielded cultures dominated by oval to round-ended rod-shaped cells. Strain 10succ1, obtained in pure culture, was characterized in detail. The non-motile cells possessed a gram-negative cell wall and did not form spores. Carbohydrates were fermented to formate, acetate, ethanol, and lactate. Succinate was decarboxylated to propionate. Other organic and amino acids were variously fermented to formate, acetate, propionate, and butyrate. Sulfur, sulfate, thiosulfate, and nitrate were not used as electron acceptors. Growth required the presence of yeast extract and at least 5 g/l NaCl, and was possible only in the absence of oxygen. No cytochromes were detected. The DNA base ratio was 40 mol% G + C. Phylogenetically, strain 10succ1 is closely related to Propionigenium modestum, as revealed by 16S rDNA analysis, but is physiologically distinct. Accordingly, strain 10succ1 (DSM 9537) is described as the type strain of a new species of the genus Propionigenium, P. maris sp. nov.
在缺氧条件下，利用潮间带泥滩沉积物中的琥珀酸盐和酵母提取物进行富集培养，得到了以椭圆形至圆形末端的杆状细胞为主的培养物。对纯培养获得的菌株10succ1进行了详细表征。该不运动细胞具有革兰氏阴性细胞壁，不形成孢子。碳水化合物发酵生成甲酸盐、乙酸盐、乙醇和乳酸盐。琥珀酸盐脱羧生成丙酸盐。其他有机化合物和氨基酸发酵生成甲酸盐、乙酸盐、丙酸盐和丁酸盐。硫、硫酸盐、硫代硫酸盐和硝酸盐不作为电子受体。生长需要酵母提取物的存在和至少5 g/l的NaCl，并且只能在无氧条件下生长。未检测到细胞色素。DNA碱基比为40 mol% G + C。16S rDNA分析表明，菌株10succ1在系统发育上与适度丙酸杆菌密切相关，但在生理上有所不同。因此，菌株10succ1（DSM 9537）被描述为丙酸杆菌属一个新物种的模式菌株，即海生丙酸杆菌新种（Propionigenium maris sp. nov.）。
