Yoshikawa H, Kuwayama N, Enose Y
Department of Biology, Faculty of Science, Nara Women's University, Japan.
J Eukaryot Microbiol. 1995 Jan-Feb;42(1):70-4. doi: 10.1111/j.1550-7408.1995.tb01542.x.
The carbohydrates of Blastocystis hominis were detected by histochemical techniques using light and electron microscopy. B. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-Schiff (PAS) or alcian blue (AB). Intense PAS reactions were observed in cells fixed with glutaraldehyde or 1/2 Karnovsky fixative. The cells fixed with other fixatives showed weak or no reactions with PAS staining. Similar results were seen in the case of AB stain. These results indicated that, depending on the fixative used, B. hominis contained PAS- or AB-reactive carbohydrates. At the electron microscopic level, ultrathin sections of B. hominis were stained with periodic acid methenamine silver (PA-MS) or periodic acid thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining techniques. Intense, positive reactions with PA-MS or PA-TCH-SP were observed on the central vacuole, Golgi apparatus, and cytoplasmic vesicles. The filamentous layer showed moderate reactions with PA-MS, whereas in PA-TCH-SP stain, it was stained more densely. The staining intensity of the central vacuole varied from cell to cell. The presence of membrane fusions of the cytoplasmic vesicles with the central vacuole indicated the accumulation of carbohydrates in the central vacuole.
采用组织化学技术，通过光学显微镜和电子显微镜检测人芽囊原虫的碳水化合物。将人芽囊原虫用各种固定剂固定，然后用去污剂处理，再用高碘酸-希夫（PAS）或阿尔辛蓝（AB）染色。在用戊二醛或1/2 Karnovsky固定剂固定的细胞中观察到强烈的PAS反应。用其他固定剂固定的细胞对PAS染色显示弱阳性或无反应。AB染色也得到类似结果。这些结果表明，根据所用固定剂的不同，人芽囊原虫含有PAS或AB反应性碳水化合物。在电子显微镜水平，人芽囊原虫的超薄切片用高碘酸亚甲胺银（PA-MS）或高碘酸硫代碳酰肼-银蛋白（PA-TCH-SP）染色技术染色。在中央液泡、高尔基体和细胞质小泡上观察到与PA-MS或PA-TCH-SP强烈的阳性反应。丝状层对PA-MS呈中度反应，而在PA-TCH-SP染色中，染色更浓密。中央液泡的染色强度因细胞而异。细胞质小泡与中央液泡的膜融合表明碳水化合物在中央液泡中积累。
