Yoshikawa H, Satoh J, Enose Y
Department of Biology, Faculty of Science, Nara Women's University, Japan.
J Electron Microsc (Tokyo). 1995 Apr;44(2):100-3.
Localization of the lipid of Blastocystis hominis was histochemically demonstrated. B. hominis was fixed with glutaraldehyde, treated with detergents, and stained with Sudan black B or Nile blue. The central vacuole of B. hominis stained with Sudan black B showed many black droplets with great variation in staining intensity. In Nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. These results indicated the presence of neutral lipids and/or acidic lipids in the central vacuole. At the ultrastructural level, the central vacuole and some cytoplasmic vesicles showed enhanced electron density after post-fixation with imidazole-buffered osmium tetroxide solution. Great variation in the density and distribution of the electron-dense materials was observed in the central vacuole. Since the electron-dense vesicles were frequently observed in the cytoplasm, B. hominis may accumulate the lipid in the central vacuole, the function of which is not well known.
人芽囊原虫脂质的定位通过组织化学方法得以证实。人芽囊原虫用戊二醛固定，经去污剂处理，并用苏丹黑B或尼罗蓝染色。用苏丹黑B染色的人芽囊原虫中央液泡显示出许多黑色液滴，染色强度差异很大。在尼罗蓝染色中，中央液泡显示出许多粉红色颗粒或均匀的蓝色反应。这些结果表明中央液泡中存在中性脂质和/或酸性脂质。在超微结构水平上，用咪唑缓冲四氧化锇溶液后固定后，中央液泡和一些细胞质囊泡显示出增强的电子密度。在中央液泡中观察到电子致密物质的密度和分布有很大差异。由于在细胞质中经常观察到电子致密囊泡，人芽囊原虫可能将脂质积累在中央液泡中，其功能尚不清楚。
