Tanaka A, Hisanaga A, Hirata M, Omura M, Makita Y, Inoue N, Ishinishi N
Department of Hygiene, Faculty of Medicine, Kyushu University, Fukuoka.
Fukuoka Igaku Zasshi. 1995 Feb;86(2):65-73.
Chronic toxicity of tar from heavy-duty diesel exhaust (HD tar) was studied in male Syrian golden hamsters which received 15 mg, 7.5 mg or 1.5 mg of HD tar as the total dosages by intratracheal instillations once a week for 15 weeks. As a control group, hamsters were treated with the 0.1 ml of Tween 60: ethanol: phosphate buffer (pH 6.88, 0.25 M) solution (5.8: 8.7: 100 by volume) once a week in the same manner. The survival rate during the instillation period in the group given 15 mg of HD tar, the high-dose group of HD tar, was the lowest, and the effect was dose-dependent. However, the survival rates during the subsequent observation period showed no marked differences among HD tar treated groups. During their total life span, one papilloma in the larynx was seen in the 44 hamsters in the group given 1.5 mg of HD tar, one papilloma in the larynx was appeared in the 59 hamsters in the group given 1.5 mg of HD tar and one lung adenoma was developed in the 58 hamsters in the control group. There were no tumors in the respiratory tract in the group given 7.5 mg of HD tar. Concerning the histopathological findings of the lung, the incidence of alveolar cell or bronchiolar cell hyperplasia in the group given 1.5 mg of HD tar was significantly higher than that in the control group. From these results, although we could not observe any tumorigenicity or carcinogenic effect of HD tar, it would seem that HD tar caused weak but positive damage to the lungs of hamsters.
在雄性叙利亚金黄地鼠中研究了重型柴油废气焦油（HD焦油）的慢性毒性，这些地鼠每周经气管内滴注一次，共15周，分别接受15毫克、7.5毫克或1.5毫克的HD焦油作为总剂量。作为对照组，以同样方式每周给地鼠注射0.1毫升吐温60：乙醇：磷酸盐缓冲液（pH 6.88，0.25M）溶液（体积比为5.8：8.7：100）。在给予15毫克HD焦油的组（HD焦油高剂量组）中，滴注期的存活率最低，且该效应呈剂量依赖性。然而，在随后的观察期内，HD焦油处理组之间的存活率没有显著差异。在其整个生命周期中，给予1.5毫克HD焦油的组中的44只地鼠中有一只出现喉部乳头状瘤，给予1.5毫克HD焦油的组中的59只地鼠中有一只出现喉部乳头状瘤，对照组中的58只地鼠中有一只发生肺腺瘤。给予7.5毫克HD焦油的组呼吸道未出现肿瘤。关于肺的组织病理学发现，给予1.5毫克HD焦油的组中肺泡细胞或细支气管细胞增生的发生率显著高于对照组。从这些结果来看，虽然我们未观察到HD焦油有任何致瘤性或致癌作用，但HD焦油似乎对仓鼠的肺部造成了轻微但肯定的损害。
