Mason D J, Lopéz-Amorós R, Allman R, Stark J M, Lloyd D
School of Pure and Applied Biology, University of Wales College of Cardiff, UK.
J Appl Bacteriol. 1995 Mar;78(3):309-15. doi: 10.1111/j.1365-2672.1995.tb05031.x.
Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful.
评估了各种染料区分活细菌和非活细菌的能力。采用了两种杀菌方法：热处理或短杆菌肽处理。染色通过两种方式进行；直接在培养基中染色或在缓冲液中染色前洗涤细胞。羰花青和罗丹明123染料在活细菌和非活细菌群体之间仅表现出微小的荧光变化。氧杂萘邻酮染料（双-1,3-二丁基巴比妥酸三甲川氧杂萘邻酮）和钙荧光白都证明更有用。
