Crandall I, Guthrie N, Demers D, Sherman I W
Department of Biology, University of California, Riverside 92521, USA.
Cell Adhes Commun. 1994 Dec;2(6):503-10. doi: 10.3109/15419069409014214.
A knobless (K-) line of the FCR-3 isolate of Plasmodium falciparum was obtained by gelatin flotation. Immunofluorescent staining and immunoblots indicated that both the K- line and the K+ (knobby) line from which it was derived contained similar forms of potentially adhesive modified band 3 protein. When the K+ and K- lines were assayed for their cytoadherent and rosetting abilities the K+ line showed a high level of CD36 dependent cytoadherence, whereas the K- line demonstrated a marked pH dependent increase in rosetting. Rosetting was inhibited by the addition of peptides based on band 3 motifs, suggesting that cytoadherence and rosetting involve the same adhesin but that the presence of knobs affects whether the adherent preference of the infected erythrocyte is uninfected red cells or endothelial/C32 amelanotic melanoma cells.
通过明胶浮选获得了恶性疟原虫FCR-3分离株的无瘤（K-）株。免疫荧光染色和免疫印迹表明，K-株及其衍生而来的K+（有瘤）株都含有相似形式的潜在粘附性修饰带3蛋白。当检测K+和K-株的细胞粘附和花结形成能力时，K+株显示出高水平的CD36依赖性细胞粘附，而K-株则表现出明显的pH依赖性花结形成增加。基于带3基序的肽的添加可抑制花结形成，这表明细胞粘附和花结形成涉及相同的粘附素，但瘤的存在会影响感染红细胞的粘附偏好是未感染的红细胞还是内皮/C32无黑色素黑色素瘤细胞。
