Telfer J F, Lyall F, Norman J E, Cameron I T
Department of Obstetrics and Gynaecology, University of Glasgow, UK.
Hum Reprod. 1995 Jan;10(1):19-23. doi: 10.1093/humrep/10.1.19.
The aim of this study was to investigate the presence of nitric oxide synthase (NOS) in human uterus. Tissues were obtained at operation from 10 women undergoing hysterectomy for benign disease. In-situ hybridization was used to determine the distribution of mRNA for NOS with a 483 bp digoxigenin-labelled antisense riboprobe. Localization of NOS was detected by (i) immunocytochemistry using a monoclonal antibody raised against bovine constitutive endothelial NOS, and (ii) NADPH diaphorase, which has been suggested to co-localize with brain NOS. Messenger RNA for NOS was detected in endometrium and myometrium from nine of 10 women, predominantly in endometrial glandular epithelium and stroma and myometrial blood vessels. NOS-like immunoreactivity was seen in endometrial stroma and myometrial blood vessels, whereas NADPH diaphorase activity was localized mainly to endometrial glandular epithelium and myometrial blood vessels. These studies suggest that different forms of constitutive NOS are present in human endometrium and myometrium, and that nitric oxide may play a role in the paracrine control of the uterine vascular bed.
本研究的目的是调查一氧化氮合酶（NOS）在人子宫中的存在情况。从10名因良性疾病接受子宫切除术的女性手术中获取组织。采用原位杂交技术，用一个483 bp地高辛标记的反义核糖探针来确定NOS mRNA的分布。通过以下方法检测NOS的定位：（i）使用针对牛组成型内皮NOS产生的单克隆抗体进行免疫细胞化学检测，以及（ii）NADPH黄递酶检测，有人认为它与脑NOS共定位。在10名女性中的9名的子宫内膜和肌层中检测到了NOS的信使RNA，主要存在于子宫内膜腺上皮、基质以及肌层血管中。在子宫内膜基质和肌层血管中可见NOS样免疫反应性，而NADPH黄递酶活性主要定位于子宫内膜腺上皮和肌层血管。这些研究表明，人子宫内膜和肌层中存在不同形式的组成型NOS，并且一氧化氮可能在子宫血管床的旁分泌调控中发挥作用。
