Lakhani S R, O'Hare M J, Monaghan P, Winehouse J, Gazet J C, Sloane J P
Department of Histopathology, Royal Marsden Hospital, Sutton, Surrey.
J Clin Pathol. 1995 Feb;48(2):164-7. doi: 10.1136/jcp.48.2.164.
To study the expression of a range of cytokeratins by malignant myoepithelioma of the breast.
Immunophenotyping was carried out using a panel of antibodies on paraffin wax embedded and frozen material using immunohistochemistry and double-labelled immunofluorescence. Electron microscopy was also performed.
The tumour cells were positive for CAM 5.2, actin, vimentin, and cytokeratin 14 and negative for cytokeratins 18 and 19. Electron microscopy showed well formed desmosomes and hemidesmosomes together with pinocytic vesicles, plentiful rough endoplasmic reticulum and 6 nM microfilaments with focal densities.
The pattern of cytokeratin expression provides further evidence that tumours with a specific myoepithelial phenotype occur rarely in the breast.
研究乳腺恶性肌上皮瘤中一系列细胞角蛋白的表达情况。
采用一组抗体，通过免疫组织化学和双标记免疫荧光技术，对石蜡包埋和冷冻的材料进行免疫表型分析。同时进行电子显微镜检查。
肿瘤细胞对CAM 5.2、肌动蛋白、波形蛋白和细胞角蛋白14呈阳性反应，对细胞角蛋白18和19呈阴性反应。电子显微镜显示有结构良好的桥粒和半桥粒，以及胞饮小泡、丰富的粗面内质网和有局灶性致密物的6 nM微丝。
细胞角蛋白的表达模式进一步证明，具有特定肌上皮表型的肿瘤在乳腺中很少见。
