Wetzels R H, Kuijpers H J, Lane E B, Leigh I M, Troyanovsky S M, Holland R, van Haelst U J, Ramaekers F C
Department of Pathology, University Hospital, Nijmegen, The Netherlands.
Am J Pathol. 1991 Mar;138(3):751-63.
In normal breast tissue and in noninvasive breast carcinomas, various keratin-14 antibodies were reactive predominantly with the basal/myoepithelial cell layer, although mainly in terminal and larger ducts luminal cells sometimes also were stained. A similar reaction pattern was found with an antibody directed against keratin 17, although this antibody was more often found negative than keratin 14 in the pre-existing myoepithelial cells in intraductal carcinomas. Furthermore antibodies reactive with hyperproliferation-related keratins 6 and 16 were used. One of these (LL025) was completely negative in normal breast tissue and noninvasive breast carcinomas. However 10% of the invasive carcinomas were diffusely or focally positive with this latter antibody, while in 18 of 115 cases of invasive breast carcinomas studied, a basal cell phenotype was detected. A relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
在正常乳腺组织和非侵袭性乳腺癌中，各种角蛋白-14抗体主要与基底/肌上皮细胞层发生反应，不过在终末导管和较大导管中，管腔细胞有时也会被染色。针对角蛋白17的抗体也呈现出类似的反应模式，尽管在导管内癌中原有的肌上皮细胞中，该抗体比角蛋白14更常呈阴性。此外，还使用了与增殖相关角蛋白6和16发生反应的抗体。其中一种（LL025）在正常乳腺组织和非侵袭性乳腺癌中完全呈阴性。然而，10%的侵袭性癌对后一种抗体呈弥漫性或局灶性阳性，而在研究的115例侵袭性乳腺癌病例中，有18例检测到基底细胞表型。在基底细胞免疫染色的癌与增殖相关角蛋白之间发现了相对较高的一致性，但这些标志物与增殖标志物Ki-67之间则没有。这支持了以下结论：乳腺癌中的基底细胞可能显示出广泛增殖，并且Ki-67染色阴性并不意味着（肿瘤）细胞没有增殖。
