变应原表位的同种型相关识别及其受抗原剂量的调节。
Isotype-associated recognition of allergen epitopes and its modulation by antigen dose.
作者信息
Kolbe L, Heusser C H, Kölsch E
机构信息
Institut für Immunologie, Universität Münster, Germany.
出版信息
Immunology. 1995 Feb;84(2):285-9.
The existence of competing and blocking IgG antibodies and their interrelationship with IgE antibodies was investigated in the IgE immune response of CBA/J mice to bee venom phospholipase A2 (PLA2) and keyhole limpet haemocyanin (KLH). Minute doses of antigen induced high titres of both IgE and IgG antibodies, whereas large doses elicited high IgG responses but only weak IgE antibody titres. Immunization with minute doses of antigen led to the production of antibodies with class-associated epitope specificities (designated epitopes G for IgG and E for IgE antibodies), and therefore no competition was observed. For IgG antibody production, G epitopes were immunodominant and IgG antibodies directed to E epitopes were produced only after immunization with an increased immunogen dose. Under this condition IgG antibodies recognizing the E epitopes acted as blocking antibodies competing with IgE antibodies for binding to E epitopes, the immunodominant epitopes for the IgE response. The data demonstrate the existance of distinct immunodominant antigenic determinants for IgE and IgG antibodies on PLA2.
在CBA/J小鼠对蜂毒磷脂酶A2（PLA2）和钥孔戚血蓝蛋白（KLH）的IgE免疫反应中，研究了竞争性和阻断性IgG抗体的存在及其与IgE抗体的相互关系。微量抗原诱导产生高滴度的IgE和IgG抗体，而大剂量抗原则引发高IgG反应，但仅产生微弱的IgE抗体滴度。用微量抗原免疫导致产生具有类别相关表位特异性的抗体（分别为IgG的G表位和IgE抗体的E表位），因此未观察到竞争现象。对于IgG抗体产生，G表位具有免疫优势，只有在用增加的免疫原剂量免疫后才产生针对E表位的IgG抗体。在这种情况下，识别E表位的IgG抗体作为阻断抗体，与IgE抗体竞争结合E表位，而E表位是IgE反应的免疫优势表位。数据表明PLA2上存在针对IgE和IgG抗体的不同免疫优势抗原决定簇。
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