Kemeny D M, Harries M G, Youlten L J, Mackenzie-Mills M, Lessof M H
J Allergy Clin Immunol. 1983 May;71(5):505-14. doi: 10.1016/0091-6749(83)90469-4.
IgE antibodies to purified proteins and peptides from honeybee venom have been measured by the RAST. Trace amounts (less than 0.1%) of the major venom protein phospholipase A2 (PLA2) grossly distorted the measurement of IgE antibody to the other venom proteins, acid phosphatase (Acid P) and hyaluronidase (HYAL), and overemphasized their importance. Reduction of antigen coupled to the cellulose paper discs, which were used in the assay, diluted out the contaminating PLA2 without apparent loss in sensitivity. The reduction of disc-bound antigen increased the competition between IgE and IgG antibodies but did not affect measurement of IgE antibodies in sera taken from 35 untreated patients who had a history of general allergic reactions to bee stings. In 54% of sera from bee venom--allergic patients, the greatest IgE antibody response was to PLA2. In all, IgE antibodies to PLA2 were present in 91% of these sera. IgE antibodies to Acid P, HYAL, or melittin were present in 60%, 51%, and 31% of sera, respectively, and accounted for the highest level of binding in 17%, 17%, and 6% of these. Only 6% of sera were positive for whole venom but negative for the isolated antigens. A low level of IgE antibody was found to peptide 401 in 6% of sera. No IgE antibodies were found to apamin. While confirming the central role played by PLA2 in bee sting allergy, these results show that other venom components are also important in some patients.
已通过放射变应原吸附试验（RAST）检测了针对蜜蜂毒液中纯化蛋白质和肽的IgE抗体。痕量（小于0.1%）的主要毒液蛋白磷脂酶A2（PLA2）严重扭曲了针对其他毒液蛋白酸性磷酸酶（Acid P）和透明质酸酶（HYAL）的IgE抗体的测量，并过度强调了它们的重要性。减少与用于该测定的纤维素纸盘偶联的抗原，稀释了污染的PLA2，而灵敏度没有明显损失。盘结合抗原的减少增加了IgE和IgG抗体之间的竞争，但不影响从35名有蜜蜂蜇伤全身过敏反应病史的未治疗患者血清中IgE抗体的测量。在来自蜜蜂毒液过敏患者的54%的血清中，最大的IgE抗体反应是针对PLA2的。总体而言，这些血清中有91%存在针对PLA2的IgE抗体。针对Acid P、HYAL或蜂毒肽的IgE抗体分别存在于60%、51%和31%的血清中，并且在这些血清的17%、17%和6%中占最高结合水平。只有6%的血清对全毒液呈阳性但对分离抗原呈阴性。在6%的血清中发现针对肽401的低水平IgE抗体。未发现针对蜂毒明肽的IgE抗体。这些结果在证实PLA2在蜜蜂蜇伤过敏中起核心作用的同时，表明其他毒液成分在某些患者中也很重要。
