Grünig G, Antczak D F
James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Biol Reprod. 1995 Mar;52(3):531-9. doi: 10.1095/biolreprod52.3.531.
The distribution of four cytokines was analyzed in the endometrium and trophoblast of the horse between Days 30 and 55 of gestation. Endometrial tissues, invasive trophoblast (chorionic girdle), and noninvasive trophoblast (chorion and allantochorion) were examined separately. Cytokine expression was determined by amplification of specific mRNA via the reverse transcriptase polymerase chain reaction (RT-PCR). Messenger RNA for interleukin 2 (IL-2), interleukin 4 (IL-4), and interferon gamma (IFN gamma) was detected in endometrial tissues, unstimulated peripheral blood lymphocytes, and control kidney tissue, but not in trophoblasts. leukocytes resident in the endometrium or traversing the uterus via blood vessels might be the source of these cytokines. Endometrial tissues and invasive and noninvasive trophoblasts expressed mRNA for tumor necrosis factor alpha TNF alpha). Immuonoreactive TNF alpha protein was detected in different cell types of the endometrium and in the invasive and noninvasive trophoblast. The ubiquitous expression of TNF alpha by the endometrium and trophoblasts suggests that this cytokine might have an important role in regulating placental growth and differentiation or maternal leukocyte responses to trophoblasts. IL-2, IL-4, and IFN gamma might have important immunoregulatory roles within the endometrium.
在妊娠30至55天期间，对马的子宫内膜和滋养层中四种细胞因子的分布进行了分析。分别检查了子宫内膜组织、侵入性滋养层（绒毛膜带）和非侵入性滋养层（绒毛膜和尿囊绒膜）。通过逆转录聚合酶链反应（RT-PCR）扩增特定mRNA来测定细胞因子的表达。在子宫内膜组织、未刺激的外周血淋巴细胞和对照肾组织中检测到白细胞介素2（IL-2）、白细胞介素4（IL-4）和干扰素γ（IFNγ）的信使RNA，但在滋养层中未检测到。驻留在子宫内膜或通过血管穿过子宫的白细胞可能是这些细胞因子的来源。子宫内膜组织以及侵入性和非侵入性滋养层均表达肿瘤坏死因子α（TNFα）的mRNA。在子宫内膜的不同细胞类型以及侵入性和非侵入性滋养层中检测到免疫反应性TNFα蛋白。子宫内膜和滋养层中TNFα的普遍表达表明，这种细胞因子可能在调节胎盘生长和分化或母体白细胞对滋养层的反应中起重要作用。IL-2、IL-4和IFNγ可能在子宫内膜内具有重要的免疫调节作用。
