Silveira J N, Heneine I F, Beirão P S
Department of Biochemistry and Immunology, Institute of Biological Sciences-UFMG, Belo Horizonte, MG, Brazil.
Toxicol Lett. 1995 Apr;76(3):187-93. doi: 10.1016/0378-4274(95)80002-u.
Fraction T2 from Tityus serrulatus venom produced a marked lengthening of action potentials recorded with the single sucrose-gap technique, a characteristic effect of alpha scorpion toxins. This effect was not reversed by thorough washing of the nerve. On the other hand, T2 fraction deactivated by complete iodination did not cause any alteration of the compound action potential, even if applied in concentration as high as 4000 times the half saturation dose of the unmodified T2 fraction. This high dose of deactivated T2 did not hinder the onset of the full effect of a single dose of T2 fraction applied subsequently. Polyclonal antibodies against native or against iodinated venom reverted the action of T2 fraction, restoring normal electrical response. We conclude that both types of antibodies may remove the effect of alpha toxins on sodium channel.
来自锯齿脂鲤属毒液的T2组分，采用单蔗糖间隙技术记录时，使动作电位显著延长，这是α型蝎毒素的典型效应。通过彻底冲洗神经，这种效应并未逆转。另一方面，经完全碘化失活的T2组分，即使以高达未修饰T2组分半饱和剂量4000倍的浓度应用，也不会引起复合动作电位的任何改变。这种高剂量的失活T2并不妨碍随后应用的单剂量T2组分发挥完全效应。针对天然毒液或碘化毒液的多克隆抗体可逆转T2组分的作用，恢复正常电反应。我们得出结论，这两种类型的抗体均可消除α毒素对钠通道的作用。
