Chávez-Olórtegui C, Fonseca S C, Campolina D, Amaral C F, Diniz C R
Centro de Pesquisas e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Toxicon. 1994 Dec;32(12):1649-56. doi: 10.1016/0041-0101(94)90323-9.
An ELISA was developed for identification of circulating toxic antigens from Tityus serrulatus scorpion venom. The toxic fraction from the scorpion venom was purified by Sephadex G-50 chromatography and immunoaffinity techniques were used for identifying antibodies that reacted with this fraction. These antibodies were used to develop a sandwich-type ELISA. The specificity of the assay was demonstrated by its capacity for identifying mice that were experimentally inoculated with T. serrulatus venom from those inoculated with Phoneutria nigriventer spider venom, Apis mellifera bee venom and Bothrops atrox, Crotalus durissus terrificus, Lachesis muta muta and Micrurus frontalis snake venoms. Measurable absorbance signals were obtained with 0.1 ng of venom per assay. The ELISA also detected antigens in the sera of patients systemically envenomed by T. serrulatus. Therefore, this ELISA could be a valuable tool for clinicians and epidemiologists, owing to its sensitivity and specificity.
开发了一种酶联免疫吸附测定法（ELISA），用于鉴定来自巴西金幽灵蝎毒液的循环毒性抗原。通过葡聚糖凝胶G-50柱色谱法纯化蝎毒中的毒性成分，并使用免疫亲和技术鉴定与该成分发生反应的抗体。这些抗体被用于开发一种夹心型ELISA。该检测方法的特异性通过其能够区分实验接种巴西金幽灵蝎毒液的小鼠与接种黑腹盗蛛毒液、意大利蜜蜂毒液以及南美巨蝮、三色矛头蝮、巨蝮和珊瑚蛇蛇毒的小鼠得以证明。每次检测使用0.1纳克毒液即可获得可测量的吸光度信号。该ELISA还检测到了被巴西金幽灵蝎全身中毒的患者血清中的抗原。因此，由于其敏感性和特异性，这种ELISA可能成为临床医生和流行病学家的一项有价值的工具。
