Honda S, Fujioka T, Shiota K, Fujiyama K, Kubota T, Murakami K, Nasu M
Second Department of Internal Medicine, Oita Medical University, Japan.
J Gastroenterol. 1995 Apr;30(2):231-6. doi: 10.1007/BF02348670.
To study the effects of ethanol on disulfiram-treated rats, we administered ethanol orally at a does of 2000 mg/kg, twice daily for 5 days. The administration of ethanol or disulfiram alone produced no recognizable changes in pancreatic acinar cells. Ethanol administration, in disulfiram-treated rats resulted in a decrease in the content of zymogen granules in acinar cells, and the appearance of intraplasmic vacuolization. Electron microscopically, these vacuoles appeared on the basal side of nuclei. In addition, similar vacuoles appeared in liver cells, and these vacuolizations seemed to show lipid inclusions. However, ethanol administration to disulfiram-treated rats did not cause inflammatory changes or edema in the pancreas. A comparison of blood ethanol levels in rats receiving ethanol alone and disulfiram plus ethanol showed no significant difference, but acetaldehyde levels in rats receiving ethanol plus disulfiram rats were significantly higher than those in rats receiving ethanol alone. These findings suggested that acetaldehyde caused a decrease of zymogen granules and the presence of lipid inclusions in pancreatic acinar cells.
为研究乙醇对双硫仑处理大鼠的影响，我们以2000毫克/千克的剂量口服给予乙醇，每日两次，共5天。单独给予乙醇或双硫仑对胰腺腺泡细胞未产生可识别的变化。在双硫仑处理的大鼠中给予乙醇导致腺泡细胞中酶原颗粒含量减少，并出现胞浆内空泡化。电子显微镜下，这些空泡出现在细胞核的基底侧。此外，肝细胞中也出现了类似的空泡，这些空泡化似乎显示为脂质包涵体。然而，对双硫仑处理的大鼠给予乙醇并未引起胰腺的炎症变化或水肿。单独接受乙醇的大鼠与接受双硫仑加乙醇的大鼠的血液乙醇水平比较无显著差异，但接受乙醇加双硫仑的大鼠的乙醛水平显著高于单独接受乙醇的大鼠。这些发现表明，乙醛导致胰腺腺泡细胞中酶原颗粒减少和脂质包涵体的存在。
