Mühler A, Zhang X, Wang H, Lawaczeck R, Weinmann H J
Research Laboratory, Schering AG, Berlin, Germany.
Invest Radiol. 1995 Feb;30(2):98-103. doi: 10.1097/00004424-199502000-00006.
The current study was designed to investigate the lymph node accumulation mechanisms of dextran-coated ultrasmall superparamagnetic iron oxide particles (USPIO) in rats.
The iron deposition in the lymph nodes after intravenous administration of USPIO at a dose of 200 mumol Fe/kg was measured in vitro by inductively coupled plasma atomic emission spectrometer in control rats, in rats after depletion of complement C3, after induction of antidextran antibodies, and with prior ligation of afferent lymphatic vessels. The results were correlated with baseline iron concentration and histology.
A significant increase in iron concentration but unequal distribution between central and peripheral nodes occurred after administration of USPIO in rats. Much less accumulation was observed in guinea pigs. In rats, the nodal uptake of USPIO was not impaired by depletion of complement C3 using cobra venom factor. The central lymph nodes (mesenteric nodes) showed significantly more accumulation of iron particles in the presence of antidextran antibodies induced by dextran preimmunization. Afferent lymphatic vessel ligation did not effect iron particle accumulation.
Accumulation of USPIO in lymph nodes is largely species-dependent but is independent of afferent lymphatic flow and of C3 complement opsonization in plasma. However, regional distribution of particles can be influenced by preimmunization using dextran.
本研究旨在探讨葡聚糖包被的超小超顺磁性氧化铁颗粒（USPIO）在大鼠体内的淋巴结蓄积机制。
通过电感耦合等离子体原子发射光谱仪，在体外测量对照组大鼠、补体C3耗竭后的大鼠、抗葡聚糖抗体诱导后的大鼠以及预先结扎输入淋巴管的大鼠静脉注射剂量为200μmol Fe/kg的USPIO后淋巴结中的铁沉积情况。将结果与基线铁浓度和组织学进行关联分析。
给大鼠注射USPIO后，铁浓度显著升高，但中央淋巴结和外周淋巴结之间的分布不均。在豚鼠中观察到的蓄积要少得多。在大鼠中，使用眼镜蛇毒因子耗竭补体C3不会损害USPIO在淋巴结中的摄取。在由葡聚糖预免疫诱导的抗葡聚糖抗体存在的情况下，中央淋巴结（肠系膜淋巴结）显示出铁颗粒的蓄积明显更多。输入淋巴管结扎不影响铁颗粒的蓄积。
USPIO在淋巴结中的蓄积在很大程度上依赖于物种，但与输入淋巴流以及血浆中的C3补体调理作用无关。然而，颗粒的区域分布可受葡聚糖预免疫的影响。
